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Recent years have seen an enormous increase in the use of protein reagents in the biological and medical sciences. Enzyme conjugates and immobilised proteins are fundamental to many diagnostic applications, and existing therapeutic advances are being made using a range of approaches based on antibody conjugates. Protein conjugation techniques are central to the development of these reagents.This may cause the solution to take on an electrophoretic mobility of its own, which can override the mobility of the solutes within it. ... Polyacrylamide gels do not contain charged groups and are therefore free of electroendosmosis problems. ... which they contain: a 3% gel will allow the free passage of solutes with a molecular mass greater than 100kDa, for example, ... While the use of gels for electrophoresis reduces theimpact of molecular diffusion and convection currents, theseanbsp;...

Author: Mohammed Aslam, Alastair Dent
Publisher: - 1998

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